Coding

Part:BBa_K2996502:Design

Designed by: Shifeng Jiang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-03)


C-luciferase is put downstream J23119


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 302
    Illegal AgeI site found at 441
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It could be linked to part BBa_K2996501,a zincfinger protein with flag tag and rrnB T1 terminator,to make a fused protein called C-lucferase-DBD.The zincfinger could bind to its target DNA sequence and bring the C-luciferase close to the N-luciferase,which also could bind to its target through dCas9.


Source

E.coli

References